Hydrogen Exchange-mass Spectrometry (HX-MS) along the Biotherapeutic Pipeline from Discovery to Biosimilars


Seminar

Date

Time

11:00 a.m.

Location

Zoom

Presenter

Roberto N. De Guzman (Professor, Department of Mulecular Biosciences, University of Kansas)

Abstract

Nanoinjectors are essential in the pathogenesis of many Gram-negative bacteria that cause infectious diseases in humans.  Nanoinjector is the structural component of a bacterial virulence mechanism, the type III secretion system (T3SS).  Examples of bacteria that rely on the T3SS for virulence are SalmonellaShigellaYersiniaPseudomonas, and Chlamydia; and they cause infectious diseases such as food poisoning, dysentery, plague, lung infections, and sexually transmitted disease.  Nanoinjectors are assembled from over 20 different proteins, forming a 4 megadalton macromolecular machinery that puncture the host cell membrane and pump virulence proteins into the host cell cytoplasm to initiate infection.  Understanding how nanoinjectors are assembled is important because of their critical role in pathogenesis.  In this seminar, I will discuss our NMR studies of proteins, including unfolded proteins and membrane proteins, that are involved in the assembly of bacterial nanoinjectors.